Coding

Part:BBa_K1491024:Design

Designed by: Danielle Peters   Group: iGEM14_Carnegie_Mellon   (2014-09-19)


ER Intein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 983
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1408
    Illegal AgeI site found at 1372
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 883


Design Notes

Estrogen sensitive intein


Source

Chong, Shaorong, Kay S. Williams, Chad Wotkowicz, and Ming-Qun Xu. "Modulation of Protein Splicing of the Saccharomyces Cerevisiae Vacuolar Membrane ATPase Intein." Journal of Biological Chemistry 273.17 (1998): 10567-0577.

References